(145) Is LIM-15 an Important Marker in Patients Who Performed Second Micro–testicular Sperm Extraction after Failed First Micro–testicular Sperm Extraction in Men with Nonobstructive Azoospermia?

Abstract Introduction LIM15, also known as DNA meiotic recombinase 1 (DMC1), is a meiosis-specific recombinase. LIM15 specifically expressed recombination protein in spermatogenesis. Objective Does the gene expression value measured ejaculate predict success of second micro–testicular sperm extraction (mTESE) after failed first mTESE? Methods Data from 111 men who mTESE and underwent between August 2018 March 2022 were prospectively collected. level was determined by real-time PCR materials ejaculate. Results Sperm recovered successfully 23 out (20.7%) with mTESE. The mean age for whom could not be retrieved successful recovery 34.6 ± 3.5 33.7 5.2, respectively. patient age, serum total testosterone FSH levels, testicular volume did show any statistical difference (Table 1). According to histology, sertoli cell-only, maturation arrest, hypospermatogenesis 26.1%, 65.2%, 8.7%, 0%, 27.3%, 72.7%. Comparing groups terms histology demonstrated meaningful correlation retrieval rate (SRR) testis (p<0.001). significantly correlated SRR (p = 0.039). As shown Figure 1, maximal sensitivity (50%) specificity (87%) obtained cutoff point 4.61 AUC 0.65. Conclusions our results, before can provide information on reduce potential complications unnecessary costs associated assisted reproductive techniques. Disclosure No